Anti-Nucleophosmin antibody [FC82291] (ab10530)

Overview

• Product nameAnti-Nucleophosmin antibody [FC82291]
  See all Nucleophosmin primary antibodies
• Description
  Mouse monoclonal [FC82291] to Nucleophosmin
• SpecificityDetects both the phosphorylated and the unphosphorylated B23 molecule.
• Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WB, ELISAmore details
• Species reactivity
  Reacts with: Mouse, Rat, Donkey, Hamster, Cow, Dog, Human, African green monkey
  
• Immunogen

  Full length native protein (purified) (Rat).

• EpitopeThe epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.
• Positive control
  • NIH3T3 whole cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 1% BSA, 0.01M PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• ClonalityMonoclonal
• Clone numberFC82291
• IsotypeIgG1
• Research areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • Regulation

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
• Positive Controls
  • NIH 3T3 whole cell lysate (ab7179)

Applications

Target

• FunctionInvolved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
• Involvement in diseaseA chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
  A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
  A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
  Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
• Sequence similaritiesBelongs to the nucleoplasmin family.
• Post-translational
  modificationsAcetylated at C-terminal lysine residues, thereby increasing affinity to histones.
  ADP-ribosylated.
  Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
  Sumoylated by ARF.
• Cellular localizationNucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.

• Target information above from: UniProt accession P06748 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 4869 Human
  • Entrez Gene: 18148 Mouse
  • Entrez Gene: 25498 Rat
  • Omim: 164040 Human
  • SwissProt: P06748 Human
  • SwissProt: Q61937 Mouse
  • SwissProt: P13084 Rat
  • Unigene: 557550 Human

  • Unigene: 485384 Mouse
  • Unigene: 54537 Rat

  see all
• Alternative names
  • B23 antibody
  • MGC104254 antibody
  • NO38 antibody

  • NPM antibody
  • NPM_HUMAN antibody
  • NPM1 antibody
  • Nucleolar phosphoprotein B23 antibody
  • Nucleolar protein NO38 antibody
  • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
  • Nucleophosmin antibody
  • nucleophosmin nucleoplasmin family member 1 antibody
  • Nucleophosmin/nucleoplasmin family member 1 antibody
  • Numatrin antibody
  • OTTHUMP00000161024 antibody
  • OTTHUMP00000161025 antibody
  • OTTHUMP00000223397 antibody
  • OTTHUMP00000223398 antibody

  see all