Synthetic phosphopeptide corresponding to residues surrounding Theonine 199 of Human Nucleophosmin
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab81551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ab81551 staining Nucleophosmin (phospho T199) in human HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton x100 in PBS and blocking with 5% serum was performed for 30 minutes at 230C. Samples were incubated with primary antibody (1/150 in PBS) for 1 hour at 23°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.