Overview

  • Product nameAnti-Nucleoporin p62 antibody
    See all Nucleoporin p62 primary antibodies
  • Description
    Mouse monoclonal to Nucleoporin p62
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LQHADEEREK TYKLAENIDA QLKRMAQDLK DIIEHLNTSG APADTSDPLQ QICKILNAHM DSLQWIDQNS ALLQRKVEEV TKVCEGRRKE QERSFRITFD , corresponding to amino acids 423-523 of Human Nucleoporin p62

Properties

Applications

Our Abpromise guarantee covers the use of ab56982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
    • Cellular localizationNucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
    • Database links
    • Alternative names
      • 62 kDa nucleoporin antibody
      • DKFZp547L134 antibody
      • FLJ20822 antibody
      • FLJ43869 antibody
      • IBSN antibody
      • MGC841 antibody
      • Nuclear pore glycoprotein p62 antibody
      • nucleoporin 62kDa antibody
      • Nucleoporin Nup62 antibody
      • nucleoporin p62 antibody
      • nucleoporin p62KD antibody
      • NUP62 antibody
      • NUP62 protein antibody
      • p62 antibody
      • SNDI antibody
      see all

    Anti-Nucleoporin p62 antibody images



    • Predicted band size : 53 kDa
      Nucleoporin p62 antibody (ab56982) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-Nucleoporin p62 antibody (ab56982)

    This product has been referenced in:
    • Zentner GE  et al. CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis. Hum Mol Genet 19:3491-501 (2010). WB ; Human . Read more (PubMed: 20591827) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (OVCA 433 ovarian cancer celll line)
    Loading amount 25 µg
    Specification OVCA 433 ovarian cancer celll line
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 12 2011

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab56982 in immunohistochemistry. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"