Overview

  • Product name
    Anti-Nucleoporin p62 antibody
    See all Nucleoporin p62 primary antibodies
  • Description
    Rabbit polyclonal to Nucleoporin p62
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 294 - 502 of Human Nucleoporin p62 (NP_057637).

  • Positive control
    • WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell

Properties

Applications

Our Abpromise guarantee covers the use of ab96134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
  • Cellular localization
    Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
  • Database links
  • Alternative names
    • 62 kDa nucleoporin antibody
    • DKFZp547L134 antibody
    • FLJ20822 antibody
    • FLJ43869 antibody
    • IBSN antibody
    • MGC841 antibody
    • Nuclear pore glycoprotein p62 antibody
    • nucleoporin 62kDa antibody
    • Nucleoporin Nup62 antibody
    • nucleoporin p62 antibody
    • nucleoporin p62KD antibody
    • NUP62 antibody
    • NUP62 protein antibody
    • p62 antibody
    • SNDI antibody
    see all

Images

  • All lanes : Anti-Nucleoporin p62 antibody (ab96134) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 53 kDa
    10% SDS PAGE
  • ab96134, at a 1/200 dilution, staining Nucleoporin p62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
    Lower image: merged with DNA probe.
  • ab96134 staining Nucleoporin p62 in mouse fore brain tissue by immunohistochemical analysis.

  • Immunofluorescence analysis of A431 cells, staining Nucleoporin p62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.

References

This product has been referenced in:
  • Ouyang F  et al. HMGB1 induces apoptosis and EMT in association with increased autophagy following H/R injury in cardiomyocytes. Int J Mol Med 37:679-89 (2016). WB . Read more (PubMed: 26847839) »
  • Lund PJ  et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). Human . Read more (PubMed: 27655845) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - nuclear (MEF)
Specification
MEF
Blocking step
Blocking One (nacalai tesque) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

教郎 鈴木

Verified customer

Submitted Dec 17 2013

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cultured Cells (U2OS)
Specification
U2OS
Permeabilization
Yes - 0.5% NP40
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Loading amount
25 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

Submitted May 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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