Anti-Nucleoside phosphorylase antibody (ab154257)

Overview

  • Product name
    Anti-Nucleoside phosphorylase antibody
    See all Nucleoside phosphorylase primary antibodies
  • Description
    Rabbit polyclonal to Nucleoside phosphorylase
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 83-289 of Human Nucleoside phosphorylase (Uniprot ID: P00491).

  • Positive control
    • Raji whole cell lysate; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Involvement in disease
    Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
  • Sequence similarities
    Belongs to the PNP/MTAP phosphorylase family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ94043 antibody
    • FLJ97288 antibody
    • FLJ97312 antibody
    • Inosine phosphorylase antibody
    • Inosine-guanosine phosphorylase antibody
    • MGC117396 antibody
    • MGC125915 antibody
    • MGC125916 antibody
    • NP antibody
    • Np1 antibody
    • Nucleoside phosphorylase antibody
    • PNP antibody
    • Pnp1 antibody
    • PNPH_HUMAN antibody
    • PRO1837 antibody
    • PUNP antibody
    • Purine nucleoside orthophosphate ribosyltransferase antibody
    • Purine nucleoside phosphorylase 5a antibody
    • Purine nucleoside phosphorylase antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nucleoside phosphorylase with ab154257 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
  • Anti-Nucleoside phosphorylase antibody (ab154257) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 32 kDa

References

ab154257 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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