Recombinant
RabMAb

Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)

Overview

  • Product name
    Anti-Nucleoside phosphorylase antibody [EPR5715]
    See all Nucleoside phosphorylase primary antibodies
  • Description
    Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Nucleoside Phosphorylase.

  • Positive control
    • Jurkat, JAR, K562, Human placenta, and 293T lysates; SH SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.76 x 10 -10 M
    Learn more about KD
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5715
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Involvement in disease
      Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
    • Sequence similarities
      Belongs to the PNP/MTAP phosphorylase family.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ94043 antibody
      • FLJ97288 antibody
      • FLJ97312 antibody
      • Inosine phosphorylase antibody
      • Inosine-guanosine phosphorylase antibody
      • MGC117396 antibody
      • MGC125915 antibody
      • MGC125916 antibody
      • NP antibody
      • Np1 antibody
      • Nucleoside phosphorylase antibody
      • PNP antibody
      • Pnp1 antibody
      • PNPH_HUMAN antibody
      • PRO1837 antibody
      • PUNP antibody
      • Purine nucleoside orthophosphate ribosyltransferase antibody
      • Purine nucleoside phosphorylase 5a antibody
      • Purine nucleoside phosphorylase antibody
      see all

    Images

    • All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : JAR lysate
      Lane 3 : K562 lysate
      Lane 4 : Human placenta lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 32 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • ab109447 at 1/100 dilution staining Nucleoside phosphorylase in SH SY5Y cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab109447 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109447.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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