Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)


  • Product nameAnti-Nucleoside phosphorylase antibody [EPR5715]
    See all Nucleoside phosphorylase primary antibodies
  • Description
    Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Nucleoside Phosphorylase.

  • Positive control
    • Jurkat, JAR, K562, Human placenta, and 293T lysates; SH SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab109447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • Involvement in diseaseDefects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
    • Sequence similaritiesBelongs to the PNP/MTAP phosphorylase family.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ94043 antibody
      • FLJ97288 antibody
      • FLJ97312 antibody
      • Inosine phosphorylase antibody
      • Inosine-guanosine phosphorylase antibody
      • MGC117396 antibody
      • MGC125915 antibody
      • MGC125916 antibody
      • NP antibody
      • Np1 antibody
      • Nucleoside phosphorylase antibody
      • PNP antibody
      • Pnp1 antibody
      • PNPH_HUMAN antibody
      • PRO1837 antibody
      • PUNP antibody
      • Purine nucleoside orthophosphate ribosyltransferase antibody
      • Purine nucleoside phosphorylase 5a antibody
      • Purine nucleoside phosphorylase antibody
      see all

    Anti-Nucleoside phosphorylase antibody [EPR5715] images

    • All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : JAR lysate
      Lane 3 : K562 lysate
      Lane 4 : Human placenta lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 32 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • ab109447 at 1/100 dilution staining Nucleoside phosphorylase in SH SY5Y cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)

    ab109447 has not yet been referenced specifically in any publications.

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