The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
FunctionMay be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
Tissue specificityIncreased levels in lung tissue in cancer patients.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family. Contains 1 G (guanine nucleotide-binding) domain.
DomainThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis. In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Cellular localizationNucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.