The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Use at 1-4 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
Tissue specificityIncreased levels in lung tissue in cancer patients.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family. Contains 1 G (guanine nucleotide-binding) domain.
DomainThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis. In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Cellular localizationNucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling Nucleostemin with ab70345 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Nucleostemin antibody (ab70345)
All lanes : Anti-Nucleostemin antibody (ab70345) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : mouse 3T3 whole cell lysate at 50 µg/ml
Predicted band size : 62 kDa Observed band size : 62 kDa
Detection of Human Nucleostemin by Western Blot of Immunprecipitate.
All lanes: ab70345 at 1µg/ml staining Nucleostemin in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: an irrelevant antibody
Lane 2: ab70345
Detection: Chemiluminescence with exposure times of 10
References for Anti-Nucleostemin antibody (ab70345)
has not yet been referenced specifically in any publications.
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