Overview

  • Product name
    Anti-Nucleostemin antibody
    See all Nucleostemin primary antibodies
  • Description
    Rabbit polyclonal to Nucleostemin
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and the C terminus (residue 549) of human Nucleostemin (accession number: NP_055181.3)

  • Positive control
    • Human skin basal cell carcinoma; mouse squamous cell carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.

Target

  • Function
    May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • Tissue specificity
    Increased levels in lung tissue in cancer patients.
  • Sequence similarities
    Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • Domain
    The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Images

  • Detection of Human Nucleostemin in formalin-fixed paraffin-embedded section of human skin basal cell carcinoma by Immunohistochemistry using ab84363 at a dilution of 1:500. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
  • Detection of Human Nucleostemin in formalin-fixed paraffin-embedded section of section of mouse squamous cell carcinoma by Immunohistochemistry using ab84363 at a dilution of 1:100. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.

References

ab84363 has not yet been referenced specifically in any publications.

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