The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.
FunctionMay be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
Tissue specificityIncreased levels in lung tissue in cancer patients.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family. Contains 1 G (guanine nucleotide-binding) domain.
DomainThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis. In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Cellular localizationNucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
Detection of Human Nucleostemin in formalin-fixed paraffin-embedded section of human skin basal cell carcinoma by Immunohistochemistry using ab84363 at a dilution of 1:500. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
Detection of Human Nucleostemin in formalin-fixed paraffin-embedded section of section of mouse squamous cell carcinoma by Immunohistochemistry using ab84363 at a dilution of 1:100. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
References for Anti-Nucleostemin antibody (ab84363)
has not yet been referenced specifically in any publications.
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