Overview

  • Product nameAnti-Nucleostemin antibody
    See all Nucleostemin primary antibodies
  • Description
    Mouse polyclonal to Nucleostemin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Nucleostemin (AAH01024.1)

  • Positive control
    • Hela and LNCaP cell lysates, Nucleostemin transfected 293T cell lysate and Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • Tissue specificityIncreased levels in lung tissue in cancer patients.
  • Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • DomainThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • Cellular localizationNucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Anti-Nucleostemin antibody images

  • Anti-Nucleostemin antibody (ab88882) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-Nucleostemin antibody (ab88882) at 1 µg/ml + LNCaP cell lysate at 50 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • All lanes : Anti-Nucleostemin antibody (ab88882) at 1 µg/ml

    Lane 1 : Nucleostemin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab88882 at 10 ug/ml staining Nucleostemin in Hela cells by Immunofluorescence

References for Anti-Nucleostemin antibody (ab88882)

ab88882 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88882.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"