Anti-Nucleus and Mitotic Cell Antigen antibody [2Q2257] (ab64608)

Overview

  • Product nameAnti-Nucleus and Mitotic Cell Antigen antibody [2Q2257]
  • Description
    Mouse monoclonal [2Q2257] to Nucleus and Mitotic Cell Antigen
  • Specificityab64608 reacts with the cell nucleus and mitotic cell antigen (poles of the mitotic spindle and adjacent micotubules, centrosomes). It is possibly related to the NuMA protein. By Western blot this antibody recognizes a major band at 210kD, preferentially in mitotic cells (additional weaker bands at 240 and 180kD).
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Live Ls 174T cells (colonic carcinoma).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone number2Q2257
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 210 kDa.
IHC-Fr Use at an assay dependent dilution.

Target

  • RelevanceThe life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M and S phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase. The Nucleus and Mitotic Cell Antigen is an antigen detected at the poles of the mitotic spindle and adjacent micotubules and centrosomes.

References for Anti-Nucleus and Mitotic Cell Antigen antibody [2Q2257] (ab64608)

ab64608 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"