Recombinant
RabMAb

Anti-NUDC antibody [EPR5072] (ab109370)

Overview

  • Product name
    Anti-NUDC antibody [EPR5072]
    See all NUDC primary antibodies
  • Description
    Rabbit monoclonal [EPR5072] to NUDC
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TRPV6.

  • Positive control
    • HepG2, 293T, HeLa, and SH-SY5Y cell lysates; Human kidney and placenta tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
    • Tissue specificity
      Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
    • Sequence similarities
      Belongs to the nudC family.
      Contains 1 CS domain.
    • Post-translational
      modifications
      Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.
    • Cellular localization
      Cytoplasm > cytoskeleton. Nucleus. In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern.
    • Information by UniProt
    • Database links
    • Alternative names
      • HNUDC antibody
      • MNUDC antibody
      • MNUDC protein antibody
      • NPD011 antibody
      • Nuclear distribution C homolog antibody
      • Nuclear distribution gene C (A.nidulans) homolog antibody
      • Nuclear distribution gene C homolog antibody
      • Nuclear distribution gene C homolog (A. nidulans) antibody
      • Nuclear distribution protein C homolog antibody
      • Nuclear migration protein nudC antibody
      • nudC antibody
      • NudC nuclear distribution protein antibody
      • NUDC_HUMAN antibody
      • OTTHUMP00000004405 antibody
      • SIG 92 antibody
      • SIG92 antibody
      see all

    Images

    • All lanes : Anti-NUDC antibody [EPR5072] (ab109370) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 83 kDa
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109370 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using ab109370 at 1/100 dilution.

    References

    ab109370 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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