Recombinant Anti-NUDC antibody [EPR5073] (ab109318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5073] to NUDC
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NUDC antibody [EPR5073]
See all NUDC primary antibodies -
Description
Rabbit monoclonal [EPR5073] to NUDC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, 293T, HeLa and SH-SY5Y cell lysates IHC-P: Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5073 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation. -
Tissue specificity
Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart. -
Sequence similarities
Belongs to the nudC family.
Contains 1 CS domain. -
Post-translational
modificationsReversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. - Information by UniProt
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Database links
- Entrez Gene: 10726 Human
- Entrez Gene: 18221 Mouse
- Entrez Gene: 29648 Rat
- Omim: 610325 Human
- SwissProt: Q9Y266 Human
- SwissProt: O35685 Mouse
- SwissProt: Q63525 Rat
- Unigene: 263812 Human
see all -
Alternative names
- HNUDC antibody
- MNUDC antibody
- MNUDC protein antibody
see all
Images
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All lanes : Anti-NUDC antibody [EPR5073] (ab109318) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : HeLa lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of NUDC in paraffin embedded Human brain tissue, using ab109318 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109318 has not yet been referenced specifically in any publications.