Recombinant
RabMAb

Anti-NUDC antibody [EPR5073] (ab109318)

Overview

  • Product name
    Anti-NUDC antibody [EPR5073]
    See all NUDC primary antibodies
  • Description
    Rabbit monoclonal [EPR5073] to NUDC
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NUDC.

  • Positive control
    • WB: HepG2, 293T, HeLa and SH-SY5Y cell lysates IHC-P: Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
  • Tissue specificity
    Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
  • Sequence similarities
    Belongs to the nudC family.
    Contains 1 CS domain.
  • Post-translational
    modifications
    Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern.
  • Information by UniProt
  • Database links
  • Alternative names
    • HNUDC antibody
    • MNUDC antibody
    • MNUDC protein antibody
    • NPD011 antibody
    • Nuclear distribution C homolog antibody
    • Nuclear distribution gene C (A.nidulans) homolog antibody
    • Nuclear distribution gene C homolog antibody
    • Nuclear distribution gene C homolog (A. nidulans) antibody
    • Nuclear distribution protein C homolog antibody
    • Nuclear migration protein nudC antibody
    • nudC antibody
    • NudC nuclear distribution protein antibody
    • NUDC_HUMAN antibody
    • OTTHUMP00000004405 antibody
    • SIG 92 antibody
    • SIG92 antibody
    see all

Images

  • All lanes : Anti-NUDC antibody [EPR5073] (ab109318) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : 293T lysate
    Lane 3 : HeLa lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of NUDC in paraffin embedded Human brain tissue, using ab109318 at a 1/250 dilution.

References

ab109318 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109318.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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