Overview

  • Product nameAnti-Nudel antibody
    See all Nudel primary antibodies
  • Description
    Rabbit polyclonal to Nudel
  • Specificityab25959 recognises Nudel.
  • Tested applicationsSuitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) - corresponding to 15 amino acids near the amino terminus of human Nudel.

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab25959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. PubMed: 17984054
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.Can be blocked with Human Nudel peptide (ab39840).

Target

  • FunctionRequired for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
  • Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Sequence similaritiesBelongs to the nudE family.
  • Developmental stageExpression peaks in mitosis.
  • Post-translational
    modifications
    Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • A. nidulans antibody
    • DKFZp451M0318 antibody
    • ENDOOLIGOPEPTIDASE A antibody
    • EOPA antibody
    • MITAP 1 antibody
    • MITAP1 antibody
    • Mitosin associated protein 1 antibody
    • Mitosin associated protein MITAP1 antibody
    • Mitosin-associated protein 1 antibody
    • Ndel 1 antibody
    • NDEL1 antibody
    • NDEL1_HUMAN antibody
    • Nuclear distribution gene E like homolog 1 antibody
    • Nuclear distribution protein nudE like 1 antibody
    • Nuclear distribution protein nudE-like 1 antibody
    • NUDE like protein antibody
    • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
    • NudE nuclear distribution gene E homolog like 1 antibody
    • NUDEL antibody
    • Protein Nudel antibody
    see all

Anti-Nudel antibody images

  • Lane 1 : Anti-Nudel antibody (ab25959) at 0.5 µg/ml
    Lane 2 : Anti-Nudel antibody (ab25959) at 1 µg/ml
    Lane 3 : Anti-Nudel antibody (ab25959) at 2 µg/ml

    Lane 1 : Jurkat whole cell lysate.
    Lane 2 : Jurkat whole cell lysate.
    Lane 3 : Jurkat whole cell lysate.


    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ab25959 at 2µg/ml staining Nudel in Jurkat cells by ICC/IF

  • Immunofluorescence of Nudel in Jurkat cells using ab25959 at 10 ug/ml.

References for Anti-Nudel antibody (ab25959)

This product has been referenced in:
  • Turgay Y  et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . Read more (PubMed: 24662567) »
  • Leliveld SR  et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008). Read more (PubMed: 18400883) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS and Hela)
Specification U2OS and Hela
Fixative Methanol
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. GUOJIE MAO

Verified customer

Submitted Dec 01 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"