The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro).
Sequence similaritiesBelongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Predominantly localized in nucleolus, and in a minor proportion in distinct foci in the nucleoplasm.