Overview

  • Product nameAnti-NUDT21 antibody
    See all NUDT21 primary antibodies
  • Description
    Rabbit polyclonal to NUDT21
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: INLYPLTNYT FGTKEPLYEK DSSVAARFQR MREEFDKIGM RRTVEGVLIV, corresponding to internal sequence amino acids 37-86 of Human NUDT21

  • Positive control
    • HepG2 cell lysate Human intestine tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500
    IHC-P: Use at a concentration of 4 - 8 µg/ml.
    WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
    • Sequence similaritiesBelongs to the Nudix hydrolase family. CPSF5 subfamily.
      Contains 1 nudix hydrolase domain.
    • Post-translational
      modifications
      Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
    • Cellular localizationNucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • CFIM 25 antibody
      • CFIM25 antibody
      • Cleavage and polyadenylation specific factor 5 25 kD subunit antibody
      • Cleavage and polyadenylation specific factor 5 25 kDa antibody
      • Cleavage and polyadenylation specific factor 5 antibody
      • Cleavage and polyadenylation specificity factor 25 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 5 antibody
      • Cleavage factor Im complex 25 kDa subunit antibody
      • CPSF 25 antibody
      • CPSF 25 kDa subunit antibody
      • CPSF 5 antibody
      • CPSF25 antibody
      • CPSF5 antibody
      • CPSF5_HUMAN antibody
      • DKFZp686H1588 antibody
      • Nucleoside diphosphate linked moiety X motif 21 antibody
      • Nucleoside diphosphate-linked moiety X motif 21 antibody
      • Nudix (nucleoside diphosphate linked moiety X) type motif 21 antibody
      • Nudix motif 21 antibody
      • NUDT 21 antibody
      • Nudt21 antibody
      • Pre mRNA cleavage factor Im (25kD) antibody
      • Pre mRNA cleavage factor Im 25 kDa subunit antibody
      • Pre mRNA cleavage factor Im 25kD subunit antibody
      • Pre-mRNA cleavage factor Im 25 kDa subunit antibody
      see all

    Anti-NUDT21 antibody images

    • All lanes : Anti-NUDT21 antibody (ab66667) at 1.25 µg/ml

      Lane 1 : Marker
      Lane 2 : HepG2 cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
      Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
      Gel concentration: 12%
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling NUDT21 with ab6667 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

    References for Anti-NUDT21 antibody (ab66667)

    ab66667 has not yet been referenced specifically in any publications.

    Product Wall

    According to our records, ab56856 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand t...

    Read More

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details, you have kindly provided and have few suggestions; these might improve the results.

    - NUDT21 as per htt...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am at...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"