Anti-NUDT22 antibody - C-terminal (ab170332)

Overview

  • Product name
    Anti-NUDT22 antibody - C-terminal
  • Description
    Rabbit polyclonal to NUDT22 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NUDT22 aa 227-257 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9BRQ3

  • Positive control
    • CEM cell line lysates, Human esophageal carcinoma; CEM cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170332 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.

Target

  • Sequence similarities
    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.
  • Domain
    Although related to the NUDIX hydrolase family, it lacks the Nudix box and does not seem to act as a hydrolase.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC13045 antibody
    • Nucleoside diphosphate-linked moiety X motif 22 antibody
    • NUD22_HUMAN antibody
    • Nudix (nucleoside diphosphate linked moiety X) type motif 22 antibody
    • Nudix motif 22 antibody
    • NUDT22 antibody
    see all

Images

  • Flow cytometric analysis of CEM cells (right/green histogram) labeling NUDT22 with ab170332 at a 1/10 dilution, compared to a negative control cell (left/blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human esophageal carcinoma labeling NUDT22  with ab170332 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-NUDT22 antibody - C-terminal (ab170332) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 33 kDa

References

ab170332 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170332.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up