Overview

  • Product nameAnti-Nuf2 antibody
    See all Nuf2 primary antibodies
  • Description
    Rabbit polyclonal to Nuf2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Chicken, Human
    Predicted to work with: Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide:

    CGGDSYAKIDEKTAELKRKMFKMS

    , corresponding to C terminal amino acids 443-463 of Human Nuf2.

  • Positive control
    • HeLa cells and cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.

Target

  • FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similaritiesBelongs to the NUF2 family.
  • Post-translational
    modifications
    Can be phosphorylated by AURKA and AURKB.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 106 antibody
    • CDCA 1 antibody
    • CDCA1 antibody
    • Cell division cycle associated 1 antibody
    • Cell division cycle associated protein 1 antibody
    • Cell division cycle-associated protein 1 antibody
    • CT106 antibody
    • hNuf 2 antibody
    • hNuf2 antibody
    • hNuf2R antibody
    • hsNuf2 antibody
    • Kinetochore protein Nuf2 antibody
    • Nuf 2 antibody
    • NUF2 antibody
    • NUF2 NDC80 kinetochore complex component antibody
    • NUF2 NDC80 kinetochore complex component homolog antibody
    • NUF2, S. cerevisiae, homolog of antibody
    • NUF2_HUMAN antibody
    • NUF2R antibody
    see all

Anti-Nuf2 antibody images

  • Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate

    Predicted band size : 54 kDa
  • ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).

References for Anti-Nuf2 antibody (ab122962)

This product has been referenced in:
  • Hu P  et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . Read more (PubMed: 25370920) »
  • Xu P  et al. B56-PP2A regulates motor dynamics for mitotic chromosome alignment. J Cell Sci 127:4567-73 (2014). WB ; Human . Read more (PubMed: 25179604) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"