Anti-Nuf2 antibody (ab122962)
Key features and details
- Rabbit polyclonal to Nuf2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nuf2 antibody
See all Nuf2 primary antibodies -
Description
Rabbit polyclonal to Nuf2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide:
CGGDSYAKIDEKTAELKRKMFKMS
, corresponding to C terminal amino acids 443-463 of Human Nuf2. -
Positive control
- HeLa cells and cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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ICC/IF | (1) |
1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining. |
Target
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Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
Sequence similarities
Belongs to the NUF2 family. -
Post-translational
modificationsCan be phosphorylated by AURKA and AURKB. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 101139481 Gorilla
- Entrez Gene: 83540 Human
- Entrez Gene: 693637 Rhesus monkey
- Omim: 611772 Human
- SwissProt: Q9BZD4 Human
- Unigene: 651950 Human
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Alternative names
- Cancer/testis antigen 106 antibody
- CDCA 1 antibody
- CDCA1 antibody
see all
Images
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Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 54 kDa -
ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab122962 has been referenced in 8 publications.
- Zhu X et al. ANP32E contributes to gastric cancer progression via NUF2 upregulation. Mol Med Rep 26:N/A (2022). PubMed: 35795988
- Papini D et al. The Aurora B gradient sustains kinetochore stability in anaphase. Cell Rep 37:109818 (2021). PubMed: 34758321
- Zhang N et al. Osteopontin accelerates the development and metastasis of bladder cancer via activating JAK1/STAT1 pathway. Genes Genomics 42:467-475 (2020). PubMed: 32088853
- Nourreddine S et al. NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay. Cell Rep 31:107660 (2020). PubMed: 32433969
- Addis Jones O et al. PLK1 facilitates chromosome biorientation by suppressing centromere disintegration driven by BLM-mediated unwinding and spindle pulling. Nat Commun 10:2861 (2019). PubMed: 31253795
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Hu P et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . PubMed: 25370920
- Xu P et al. B56-PP2A regulates motor dynamics for mitotic chromosome alignment. J Cell Sci 127:4567-73 (2014). WB ; Human . PubMed: 25179604