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Rabbit polyclonal to Nuf2 - C-terminal
Predicted to work with:
Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human Nuf2 aa 414-464 (C terminal). The exact sequence is proprietary. (NP_663735.2)
HeLa, 293T, Jurkat, and NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
Use at 2-10 µg/mg of lysate.
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Belongs to the NUF2 family.
Can be phosphorylated by AURKA and AURKB.
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Information by UniProt
Cancer/testis antigen 106 antibody
CDCA 1 antibody
Western blot - Anti-Nuf2 antibody - C-terminal (ab187682)
All lanes : Anti-Nuf2 antibody - C-terminal (ab187682) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK-1, whole cell lysate Lane 5 : NIH3T3 whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique Predicted band size : 54 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-Nuf2 antibody - C-terminal (ab187682)
Immunoprecipitation. ab187682 at 1 µg/ml staining Nuf2 in HeLa whole cell lysate immunoprecipitated using ab187682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"