Overview

  • Product name
  • Description
    Rabbit polyclonal to NuMA
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
    Predicted to work with: Mammal
  • Immunogen

    Recombinant full length protein corresponding to Human NuMA.

  • Positive control
    • HeLa whole cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab36999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP 1/10 - 1/500.

Target

  • Function
    May be a structural component of the nucleus.
  • Cellular localization
    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody images

  • ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.

References for Anti-NuMA antibody (ab36999)

This product has been referenced in:
  • Platani M  et al. Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes. J Cell Biol 210:45-62 (2015). ICC/IF ; Human . Read more (PubMed: 26124292) »
  • Kulukian A  et al. Epidermal development, growth control, and homeostasis in the face of centrosome amplification. Proc Natl Acad Sci U S A 112:E6311-20 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26578791) »

See all 13 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Specification
MDCK cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Username

Dr. Inma Banon

Verified customer

Submitted Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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