Overview

  • Product nameAnti-NuMA antibody
    See all NuMA primary antibodies
  • Description
    Rabbit polyclonal to NuMA
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Marmoset (common)
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Melanoma, Stomach Adenocarcinoma and Testicular Seminoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • FunctionMay be a structural component of the nucleus.
  • Cellular localizationNucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody images

  • ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using DAB staining.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.

References for Anti-NuMA antibody (ab84680)

This product has been referenced in:
  • Sugawa F  et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J 34:1009-24 (2015). IHC-P ; Human . Read more (PubMed: 25750208) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 25%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6,0)
Sample Marmoset (common) Tissue sections (Testis)
Specification Testis
Permeabilization Yes - Triton X-100 (1:100 in PBS) for 30 min
Fixative Buoin's fixative
Username

Abcam user community

Verified customer

Submitted Nov 26 2013

Thank you for contacting us.

At the site recognized by the antibody the human and mouse sequences are too divergent to expect binding. This antibody is not a good candidate for use in mouse although it has not been tested.

I hope this...

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Thanks for your enquiry.

Regarding the immunogen information:
ab5675 immunogen is Live Ls 174T cells and epitope is not known.

RE: could you provide Ab86129 and/or Ab84680 without BSA?
These 2 antibodies are not typically ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"