Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).
Concentration is optimized for IHC and not determined
Our Abpromise guarantee covers the use of ab84680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.