Overview

  • Product nameAnti-NuMA antibody
    See all NuMA primary antibodies
  • Description
    Rabbit polyclonal to NuMA
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).

  • Positive control
    • 293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 238 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay be a structural component of the nucleus.
  • Cellular localizationNucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody images

  • Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 238 kDa
  • ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
  • ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.

References for Anti-NuMA antibody (ab97585)

This product has been referenced in:
  • Gruber Filbin M  et al. Coordinate activation of Shh and PI3K signaling in PTEN-deficient glioblastoma: new therapeutic opportunities. Nat Med 19:1518-23 (2013). IHC ; Human . Read more (PubMed: 24076665) »
  • Gregory LS  et al. Breast cancer cells induce osteolytic bone lesions in vivo through a reduction in osteoblast activity in mice. PLoS One 8:e68103 (2013). IHC ; Human . Read more (PubMed: 24069136) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Human Tissue sections (Engineerd construct)
Specification Engineerd construct
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Hendrik Gremmels

Verified customer

Submitted Jan 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"