Anti-NUMB antibody [1A7] (ab123891)

Overview

  • Product nameAnti-NUMB antibody [1A7]
    See all NUMB primary antibodies
  • Description
    Mouse monoclonal [1A7] to NUMB
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human recombinant NUMB produced in HEK293T cells (NP_003735).

  • Positive control
    • COS7 cells transiently transfected by pCMV6-ENTRY NUMB. Lysate from HEK293 cells transiently transfected by pCMV6-ENTRY NUMB.

Properties

Applications

Our Abpromise guarantee covers the use of ab123891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 71 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • Sequence similaritiesContains 1 PID domain.
  • Post-translational
    modifications
    Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c14_5527 antibody
    • C14orf41 antibody
    • FLJ31314 antibody
    • h Numb antibody
    • h-Numb antibody
    • Numb antibody
    • Numb homolog (Drosophila) antibody
    • Numb homolog antibody
    • Numb protein homolog antibody
    • NUMB_HUMAN antibody
    • Protein numb homolog antibody
    • Protein S171 antibody
    • S171 antibody
    see all

Anti-NUMB antibody [1A7] images

  • All lanes : Anti-NUMB antibody [1A7] (ab123891) at 1/2000 dilution

    Lane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY NUMB cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216230)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB using 1/100 ab123891.
  • HEK293T cells transfected with either NUMB overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab123891 at 1/100 dilution, and then analyzed by flow cytometry.

References for Anti-NUMB antibody [1A7] (ab123891)

ab123891 has not yet been referenced specifically in any publications.

Product Wall

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Unfortunately the exact epitope sight for ab123891 has not been mapped however based on the IF protocol that was used to test the antibody, we believe that the epitope may be intracellular (as they had to use 0.1% T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"