Overview

  • Product nameAnti-NUMB antibody
    See all NUMB primary antibodies
  • Description
    Goat polyclonal to NUMB
  • SpecificityThis antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: PFSSDLQKTFEIEL, corresponding to C terminal amino acids 638-651 of Human NUMB.

  • Positive control
    • A431 lysate. Mouse brain lysates.
  • General notesThis product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. See Abreview
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human NUMB peptide (ab22990).
IP 1/1000. For 1 hr on mouse brain lysate (see Abreview).
ICC/IF Use at an assay dependent dilution. PubMed: 17127749

Target

  • FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • Sequence similaritiesContains 1 PID domain.
  • Post-translational
    modifications
    Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c14_5527 antibody
    • C14orf41 antibody
    • FLJ31314 antibody
    • h Numb antibody
    • h-Numb antibody
    • Numb antibody
    • Numb homolog (Drosophila) antibody
    • Numb homolog antibody
    • Numb protein homolog antibody
    • NUMB_HUMAN antibody
    • Protein numb homolog antibody
    • Protein S171 antibody
    • S171 antibody
    see all

Anti-NUMB antibody images

  • ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 71 kDa

    Western Blot analysis of rat brain lysate (35µg protein in RIPA buffer)labeling NUMB with ab4147 at 0.01µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

  • Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.

    This picture was kindly supplied as part of the review submitted by Christopher Mann.



  • Predicted band size : 71 kDa

    ab4147 staining (0.03µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-NUMB antibody (ab4147)

This product has been referenced in:
  • Kim KK  et al. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol 200:443-58 (2013). Chicken . Read more (PubMed: 23420872) »
  • Cox JL  et al. The SOX2-interactome in brain cancer cells identifies the requirement of MSI2 and USP9X for the growth of brain tumor cells. PLoS One 8:e62857 (2013). WB ; Human . Read more (PubMed: 23667531) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Verified customer

Submitted Mar 19 2013

Thank you for submitting an Abreview of ab4147. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on t...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin equivalent)
Specification Skin equivalent
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako EnVision Kit 5007
Permeabilization No
Blocking step (agent) for 20 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Jul 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (skin equivalent, human keratinocytes Wholemount)
Specification skin equivalent, human keratinocytes Wholemount
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton-X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jul 06 2012

Thank you for contacting us.


The anti-NUMB antibody (ab4147) is raised against a peptide corresponding to residues 638-651 of human NUMB. Through sequence alignment of the Drosophila melanogaster protein (SwissProt reference http://www....

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic brain tissue)
Specification embryonic brain tissue
Fixative Paraformaldehyde
Permeabilization Yes - 1% TX100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryo CNS)
Specification embryo CNS
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM sodiumcitrate pH 6.0, also Tris-EDTA pH 9.0 tried
Permeabilization Yes - 0.1 % Tween-20 for 10-30 minutes
Blocking step !% BSA and 10%donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Oct 26 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryo)
Specification embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
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Submitted Jul 13 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (embryo brain)
Loading amount 30 µg
Specification embryo brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 05 2007

Thank you for your enquiry and for providing me with details of the western blotting experiments that you have performed. From the internal notes that I have I can see that mouse reactivity by this antibody was added as a result of two independent A...

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