The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesTested in peptide ELISA: antibody detection limit dilution >1/32000.
Western Blot: Use at an assay dependent dilution.
We would welcome any customer feedback about ab5641.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
Sequence similaritiesContains 1 PID domain.
DomainThe PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
Cellular localizationCytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.