Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5% Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesTested in peptide ELISA: antibody detection limit dilution >1/32000.
    Western Blot: Use at an assay dependent dilution.
    We would welcome any customer feedback about ab5641.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
    • Sequence similaritiesContains 1 PID domain.
    • DomainThe PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
    • Cellular localizationCytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAG 3A antibody
      • CAG3A antibody
      • CTG 3a antibody
      • CTG3a antibody
      • NBL antibody
      • NUMB Drosophilia Homolog Like antibody
      • Numb homolog (Drosophila) like antibody
      • Numb homolog like antibody
      • Numb like protein antibody
      • NUMB R antibody
      • Numb-like protein antibody
      • Numb-R antibody
      • Numb-related gene antibody
      • Numb-related protein antibody
      • NUMBL antibody
      • NUMBL_HUMAN antibody
      • NUMBLIKE antibody
      • NUMBR antibody
      • TNRC 23 antibody
      • TNRC23 antibody
      • Trinucleotide repeat-containing gene 23 antibody
      see all

    Anti-NUMBL antibody images

    • Lanes 1 - 2 : Anti-NUMBL antibody (ab5641) at 1 µg/ml
      Lane 3 : anti-MYC Tag at 1/1000 dilution

      Lane 1 : HEK293 Lysate overexpressing Human Numbl with C-terminal MYC tag
      Lane 2 : Mock transfected HEK293
      Lane 3 : HEK293 Lysate overexpressing Human Numbl with C-terminal MYC tag

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Primary incubations were for 1 hour

    References for Anti-NUMBL antibody (ab5641)

    This product has been referenced in:
    • Tokumitsu H  et al. Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. J Biol Chem 280:35108-18 (2005). Read more (PubMed: 16105844) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"