Recombinant
RabMAb

Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Overview

  • Product name
    Anti-NUP133 antibody [EPR10808(B)]
    See all NUP133 primary antibodies
  • Description
    Rabbit monoclonal [EPR10808(B)] to NUP133
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human NUP133.

  • Positive control
    • HeLa, Ramos and SH-SY5Y cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Involved in poly(A)+ RNA transport.
    • Sequence similarities
      Belongs to the nucleoporin Nup133 family.
    • Cellular localization
      Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
    • Information by UniProt
    • Database links
    • Alternative names
      • 133 kDa nucleoporin antibody
      • FLJ10814 antibody
      • hNUP 133 antibody
      • hNUP133 antibody
      • MGC21133 antibody
      • NU133_HUMAN antibody
      • Nuclear pore complex protein Nup133 antibody
      • Nucleoporin 133kDa antibody
      • Nucleoporin NUP133 antibody
      • NUP 133 antibody
      • NUP133 antibody
      • OTTHUMP00000061095 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 129 kDa
      Observed band size: 129 kDa

    • Lanes 1 & 3 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purified)
      Lane 2 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purifed)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
      Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 129 kDa
      Observed band size: 129 kDa

    • All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 129 kDa



      This image was produced using unpurified antibody.

    • Unpurified ab155990 staining NUP133 in mokey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.

      See Abreview

    • Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.

    References

    ab155990 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Monkey Cell (Kidney)
    Specification
    Kidney
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Aaron Halpen

    Verified customer

    Submitted Sep 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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