Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)
All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Ramos cell lysate Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 129 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)This image is courtesy of an Abreview submitted by Aaron Halpen
ab155990 staining NUP133 in mokey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody.