Overview

  • Product name
  • Description
    Rabbit polyclonal to Nup153
  • Tested applications
    Suitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 50 and 100 of human Nup153 (NP_005115.2).

  • Positive control
    • Human HeLa cells
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab84872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500. (formaldehyde fixed cells). Permeabilization with Triton X 100 is recommended. Acetone fixation is not recommended.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 21858095

Target

  • Function
    Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
  • Sequence similarities
    Contains 4 RanBP2-type zinc fingers.
  • Domain
    Contains F-X-F-G repeats.
  • Cellular localization
    Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 153 kDa nucleoporin antibody
    • HNUP153 antibody
    • N153 antibody
    • NU153_HUMAN antibody
    • Nuclear pore complex protein hnup153 antibody
    • Nuclear pore complex protein Nup153 antibody
    • Nucleoporin 153kDa antibody
    • Nucleoporin Nup153 antibody
    • Nup 153 antibody
    • Nup153 antibody
    see all

Images

  • All lanes : Anti-Nup153 antibody (ab84872) at 1/1000 dilution

    Lane 1 : U2OS cell lysate - treated with DMSO
    Lane 2 : U2OS cell lysate - treated with H2O2

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye® 800CW-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 153 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Staining of human NUP153 in NBF fixed, asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
  • Staining of human NUP153 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red-fluorescent goat anti-rabbit IgG used at a dilution of 1/100.

References

This product has been referenced in:
  • Dharan A  et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog 12:e1005700 (2016). Read more (PubMed: 27327622) »
  • Qian J  et al. Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nat Commun 6:10215 (2015). Read more (PubMed: 26674376) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human smooth muscle cells)
Permeabilization
Yes - 0.5% NP-40
Specification
human smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Fixative
Paraformaldehyde
Username

Miss. Can Zhou

Verified customer

Submitted Feb 09 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - nuclear (U2OS)
Specification
U2OS
Treatment
DMSO
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - nuclear (NALM6)
Specification
NALM6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted May 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample
Human Cell (293T cells)
Specification
293T cells
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 14 2014

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cultured Cells (U2OS)
Specification
U2OS
Permeabilization
Yes - 0.5% NP40
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Loading amount
20 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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