• Product name
    Anti-Nup153 antibody [SA1]
    See all Nup153 primary antibodies
  • Description
    Mouse monoclonal [SA1] to Nup153
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig
  • Immunogen

    GST-Nup153 C-terminal domain fusion protein

  • Positive control
    • This antibody gave a positive signal in HepG2 cells (Immunocytochemistry). This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma.
  • General notes

    This antibody clone is manufactured by Abcam.

    Alternative versions available:

    Anti-Nup153 antibody [SA1] - BSA and Azide free (ab174767)
    Anti-Nup153 antibody (Alexa Fluor® 647) [SA1] (ab205845)




Our Abpromise guarantee covers the use of ab96462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration.


Anti-Nup153 antibody [SA1] images

  • ICC/IF image of ab96462 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96462, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.
  • IHC image of Nup153 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96462, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Nup153 antibody [SA1] (ab96462)

This product has been referenced in:
  • Lowe AR  et al. Importin-ß modulates the permeability of the nuclear pore complex in a Ran-dependent manner. Elife 4:N/A (2015). Read more (PubMed: 25748139) »
  • Schooley A  et al. The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis. J Cell Sci 128:3466-77 (2015). Read more (PubMed: 26224877) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HeLa cell)
Loading amount
20 µg
HeLa cell
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Jan 21 2013


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