Overview

  • Product nameAnti-NUP160 antibody
    See all NUP160 primary antibodies
  • Description
    Rabbit polyclonal to NUP160
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human NUP160.

  • Positive control
    • Extracts from HepG2 cells, NIH/3T3 cells and A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 149 kDa.
ELISA 1/20000.

Target

Anti-NUP160 antibody images

  • All lanes : Anti-NUP160 antibody (ab74147) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from NIH/3T3 cells
    Lane 3 : Extracts from A549 cells
    Lane 4 : Extracts from NIH/3T3 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 149 kDa
    Observed band size : 149 kDa
  • ab74147 (1/100) staining NUP160 in human HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). Secondary antibody was goat anti-rabbit IgG conjugated to FITC. For further experimental details please refer to Abreview.

    See Abreview

References for Anti-NUP160 antibody (ab74147)

This product has been referenced in:
  • Yokoyama H  et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun 5:3270 (2014). Read more (PubMed: 24509916) »
  • Tarazón E  et al. Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes. PLoS One 7:e48957 (2012). WB ; Human . Read more (PubMed: 23152829) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"