Overview

  • Product name
    Anti-NUP205 antibody
  • Description
    Rabbit polyclonal to NUP205
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1750-1800 of Human NUP205 (NP_055950.1).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab157090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 228 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-NUP205 antibody (ab157090) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 228 kDa


    Exposure time : 3 minutes
  • ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes NUP205
    Lane 2: ab157090 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab157090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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