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Rabbit polyclonal to NUP205
HumanPredicted to work with:
Cow, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region within amino acids 1750-1800 of Human NUP205 (NP_055950.1).
- 293T, HeLa and Jurkat whole cell lysates
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Predicted molecular weight: 228 kDa.
||Use at 2-10 µg/mg of lysate.
Nucleus > nuclear pore complex.
Information by UniProt
- 205 kDa nucleoporin antibody
- C7orf14 antibody
- KIAA0225 antibody
Western blot - Anti-NUP205 antibody (ab157090)
All lanes : Anti-NUP205 antibody (ab157090) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 228 kDa
Exposure time: 3 minutes
Immunoprecipitation - Anti-NUP205 antibody (ab157090)
ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes NUP205
Lane 2: ab157090 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"