Anti-NUP214 antibody (ab70497)
Key features and details
- Rabbit polyclonal to NUP214
- Suitable for: ICC, ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NUP214 antibody
See all NUP214 primary antibodies -
Description
Rabbit polyclonal to NUP214 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: ICC, ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residues 1250 and 1300 of human NUP214. (NP_005076.3)
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Positive control
- WB: HeLa and KEK293T lysate; IP: HeLa lysate; IHC: ovarian carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19339552
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa).
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IP | (1) |
Use at 2-10 µg/mg of lysate.
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Notes |
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ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19339552 |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. -
Tissue specificity
Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development. -
Involvement in disease
Note=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. -
Domain
Contains FG repeats. -
Post-translational
modificationsProbably glycosylated as it reacts with wheat germ agglutinin (WGA). -
Cellular localization
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 8021 Human
- Entrez Gene: 227720 Mouse
- Omim: 114350 Human
- SwissProt: P35658 Human
- SwissProt: Q80U93 Mouse
- Unigene: 654530 Human
- Unigene: 289686 Mouse
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Alternative names
- 214 kDa nucleoporin antibody
- CAIN antibody
- CAN antibody
see all
Images
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All lanes : Anti-NUP214 antibody (ab70497) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 214 kDa -
NUP214 was immunoprecipitated from HeLa whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70497 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70497 at 0.04 µg/mL.
Lane 1: ab70497 IP in HeLa whole cell lysate.Lane 2: ab70497 IP in HeLa whole cell lysate.
Lane 2: rabbit anti-NUP214 antibody IP in HeLa whole cell lysate.
Lane 4: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab70497 has been referenced in 18 publications.
- Cigdem S et al. SET-NUP214 and MLL cooperatively regulate the promoter activity of the HoxA10 gene. Genes Cells 26:830-837 (2021). PubMed: 34320268
- Senbas Akyazi B et al. Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214. Turk J Biol 44:82-92 (2020). PubMed: 32256144
- Lv L et al. Long Non-coding RNA LINC00114 Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression. Front Oncol 9:1383 (2019). PubMed: 31921641
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
- Fichtman B et al. Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy. Am J Hum Genet 105:48-64 (2019). PubMed: 31178128