Overview

  • Product nameAnti-NUP214 antibody
    See all NUP214 primary antibodies
  • Description
    Rabbit polyclonal to NUP214
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: ICC/IF, ICC, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1250 and 1300 of human NUP214. (NP_005076.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19339552
ICC 1/50 - 1/250.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
  • Tissue specificityExpressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
  • Involvement in diseaseNote=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
    Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
  • DomainContains FG repeats.
  • Post-translational
    modifications
    Probably glycosylated as it reacts with wheat germ agglutinin (WGA).
  • Cellular localizationNucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 214 kDa nucleoporin antibody
    • CAIN antibody
    • CAN antibody
    • CAN protein putative oncogene antibody
    • D9S46E antibody
    • N214 antibody
    • NU214_HUMAN antibody
    • Nuclear pore complex protein Nup214 antibody
    • nucleoporin 214kDa antibody
    • Nucleoporin Nup214 antibody
    • Nup214 antibody
    • p250 antibody
    • Protein CAN antibody
    see all

Anti-NUP214 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling NUP214 with ab70497 at 1/100 (2µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • All lanes : Anti-NUP214 antibody (ab70497) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 214 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of Human NUP214 by Western Blot of Immunprecipitate. ab70497 at 1µg/ml staining NUP214 in HeLa whole cell lysates immunoprecipitated using ab70497 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-NUP214 antibody (ab70497)

This product has been referenced in:
  • Kawaguchi A  et al. Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly. Sci Rep 5:8768 (2015). ICC/IF . Read more (PubMed: 25740062) »
  • Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB ; Human . Read more (PubMed: 22253824) »

See all 4 Publications for this product

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