Overview

  • Product nameAnti-NUP214 antibody
    See all NUP214 primary antibodies
  • Description
    Rabbit polyclonal to NUP214
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1250 and 1300 of human NUP214 (NP_005076.3).

  • Positive control
    • Human ovarian carcinoma. NBF-fixed asynchronous HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab84357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/500. Fix with formaldehyde.

Target

  • FunctionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
  • Tissue specificityExpressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
  • Involvement in diseaseNote=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
    Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
  • DomainContains FG repeats.
  • Post-translational
    modifications
    Probably glycosylated as it reacts with wheat germ agglutinin (WGA).
  • Cellular localizationNucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 214 kDa nucleoporin antibody
    • CAIN antibody
    • CAN antibody
    • CAN protein putative oncogene antibody
    • D9S46E antibody
    • N214 antibody
    • NU214_HUMAN antibody
    • Nuclear pore complex protein Nup214 antibody
    • nucleoporin 214kDa antibody
    • Nucleoporin Nup214 antibody
    • Nup214 antibody
    • p250 antibody
    • Protein CAN antibody
    see all

Anti-NUP214 antibody images

  • ab84357, at 1/100 dilution, staining NUP214 in formalin-fixed, paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry using DAB detection and an anti-Rabbit IgG HRP conjugated secondary antibody at a dilution of 1/100.
  • ab84357, at 1/250 dilution, staining NUP214 in NBF-fixed asynchronous HeLa cells by Immunofluorescence using an Anti-rabbit IgG-FITC conjugated secondary antibody at a dilution of 1/100.

References for Anti-NUP214 antibody (ab84357)

This product has been referenced in:
  • Di Nunzio F  et al. Human Nucleoporins Promote HIV-1 Docking at the Nuclear Pore, Nuclear Import and Integration. PLoS One 7:e46037 (2012). WB, ICC/IF . Read more (PubMed: 23049930) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"