The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
1/100 - 1/500. Fix with formaldehyde.
FunctionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Tissue specificityExpressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Involvement in diseaseNote=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
DomainContains FG repeats.
Post-translational modificationsProbably glycosylated as it reacts with wheat germ agglutinin (WGA).
ab84357, at 1/100 dilution, staining NUP214 in formalin-fixed, paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry using DAB detection and an anti-Rabbit IgG HRP conjugated secondary antibody at a dilution of 1/100.