Anti-NUP43 antibody (ab69447)
Key features and details
- Mouse polyclonal to NUP43
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUP43 antibody -
Description
Mouse polyclonal to NUP43 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human NUP43. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human placenta tissue lysate; NUP43 transfected 293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
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Relevance
NUP43 probably mediates the assembly of subdomains of the nuclear pore complex (NPC) or facilitates the interaction of transport complexes with the NPC. -
Cellular localization
Nucleus; nuclear pore complex. -
Database links
- Entrez Gene: 348995 Human
- Omim: 608141 Human
- SwissProt: Q8NFH3 Human
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Alternative names
- bA350J20.1 antibody
- FLJ13287 antibody
- Nucleoporin 43kDa antibody
see all
Images
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Anti-NUP43 antibody (ab69447) at 1/500 dilution + human placenta tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-NUP43 antibody (ab69447) at 1/500 dilution
Lane 1 : NUP43 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
ab69447 at 10µg/ml staining NUP43 in Hela cells by ICC/IF.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab69447 has not yet been referenced specifically in any publications.