Overview

  • Product name
  • Description
    Mouse polyclonal to NUP43
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NUP43 (NP_942590.1).

  • Positive control
    • Human placenta tissue lysate; NUP43 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Relevance
    NUP43 probably mediates the assembly of subdomains of the nuclear pore complex (NPC) or facilitates the interaction of transport complexes with the NPC.
  • Cellular localization
    Nucleus; nuclear pore complex.
  • Database links
  • Alternative names
    • bA350J20.1 antibody
    • FLJ13287 antibody
    • Nucleoporin 43kDa antibody
    • Nucleoporin Nup43 antibody
    • Nup107-160 subcomplex subunit Nup43 antibody
    • p42 antibody
    see all

Images

  • Anti-NUP43 antibody (ab69447) at 1/500 dilution + human placenta tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-NUP43 antibody (ab69447) at 1/500 dilution

    Lane 1 : NUP43 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ab69447 at 10µg/ml staining NUP43 in Hela cells by ICC/IF.

References

ab69447 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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