Overview

  • Product nameAnti-NUP50 antibody
    See all NUP50 primary antibodies
  • Description
    Goat polyclonal to NUP50
  • SpecificityThis antibody is expected to recognise all three human isoforms.
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-ELHKILLEKKDA

    , corresponding to C terminal amino acids 457-468 of Human NUP50(Peptide available as ab22982.)

  • Positive control
    • Jurkat lysate.
  • General notesPrincipal Names – NUP50; NPAP60; NPAP60L; MGC39961; DKFZP564A043; nucleoporin 50kDa; nuclear pore-associated protein 60L Official Gene Symbol - NUP50 GenBank Accession Number – NP_009103; NP_705931; NP_710151 LocusLink ID - 10762 (human); 18141 (mouse) Gene Ontology terms - nuclear pore

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2.5 - 3.8 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Can be blocked with Human NUP50 peptide (ab22982).

Target

  • FunctionComponent of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • Tissue specificityUbiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • Sequence similaritiesContains 1 RanBD1 domain.
  • DomainContains FG repeats.
  • Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Anti-NUP50 antibody images

  • ab4005 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab4005 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-NUP50 antibody (ab4005)

This product has been referenced in:
  • Broggini-Tenzer A  et al. Substrate screening identifies a novel target sequence for the proteasomal activity regulated by ionizing radiation. Proteomics 10:304-14 (2010). WB ; Mouse . Read more (PubMed: 19957288) »
  • González JM  et al. Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope. J Cell Biol 183:653-66 (2008). WB ; Mouse . Read more (PubMed: 19015316) »

See all 4 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"