Anti-NUP50 antibody (ab85915)
Key features and details
- Rabbit polyclonal to NUP50
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NUP50 antibody
See all NUP50 primary antibodies -
Description
Rabbit polyclonal to NUP50 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between residues 418 and 468 of Human NUP50 (NP_009103.2)
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Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85915 was affinity purified using an epitope specific to NUP50 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. -
Tissue specificity
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. -
Sequence similarities
Contains 1 RanBD1 domain. -
Domain
Contains FG repeats. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex. - Information by UniProt
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Database links
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- Unigene: 727643 Human
- Unigene: 28379 Mouse
- Unigene: 391486 Mouse
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Alternative names
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
see all
Images
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All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB.
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Detection of NUP50 by Western Blot of Immunprecipitate.
ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab85915 has been referenced in 1 publication.
- Holzer G et al. The nucleoporin Nup50 activates the Ran guanine nucleotide exchange factor RCC1 to promote NPC assembly at the end of mitosis. EMBO J 40:e108788 (2021). PubMed: 34725842