Overview

  • Product name
  • Description
    Rabbit polyclonal to NUP50
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 418 and 468 of Human NUP50 (NP_009103.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • Tissue specificity
    Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • Sequence similarities
    Contains 1 RanBD1 domain.
  • Domain
    Contains FG repeats.
  • Cellular localization
    Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate. at 50 µg
    Lane 2 : HeLa whole cell lysate. at 15 µg
    Lane 3 : HeLa whole cell lysate. at 5 µg


    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 30 seconds
  • Detection of NUP50 by Western Blot of Immunprecipitate.
    ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab85915 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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