Overview

  • Product nameAnti-NUP50 antibody
    See all NUP50 primary antibodies
  • Description
    Mouse polyclonal to NUP50
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human NUP50 (NP_009103.2, amino acids 1-468)

  • Positive control
    • Human liver tissue lysate, NUP50 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • FunctionComponent of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • Tissue specificityUbiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • Sequence similaritiesContains 1 RanBD1 domain.
  • DomainContains FG repeats.
  • Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Anti-NUP50 antibody images

  • Anti-NUP50 antibody (ab89524) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-NUP50 antibody (ab89524) at 1 µg/ml

    Lane 1 : NUP50 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-NUP50 antibody (ab89524)

ab89524 has not yet been referenced specifically in any publications.

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