Overview

  • Product nameAnti-NUP84 antibody
  • Description
    Goat polyclonal to NUP84
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide:

    ELSPTYQTERFTK-C

    , corresponding to N terminal amino acids 2-14 of Saccharomyces cerevisiae NUP84 according to NP_010167.1.

  • Positive control
    • Saccharomyces cerevisiae S288c lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 84 kDa).

Target

Anti-NUP84 antibody images

  • All lanes : Anti-NUP84 antibody (ab122883) at 2 µg/ml (incubation time: 1 hour)

    Lane 1 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - knock out
    Lane 2 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - wild type

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 84 kDa

References for Anti-NUP84 antibody (ab122883)

ab122883 has not yet been referenced specifically in any publications.

Product Wall

As we have no comment regarding the incubation condition, it shouldn't be a problem to use your standard lab protocol and optimize the results from that point.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"