Anti-NUP84 antibody (ab122883)
Key features and details
- Goat polyclonal to NUP84
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-NUP84 antibody -
Description
Goat polyclonal to NUP84 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Synthetic peptide:
ELSPTYQTERFTK-C
, corresponding to N terminal amino acids 2-14 of Saccharomyces cerevisiae NUP84 according to NP_010167.1. -
Positive control
- Saccharomyces cerevisiae S288c lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 84 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 84 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Database links
- Entrez Gene: 851441 Saccharomyces cerevisiae
- SwissProt: P52891 Saccharomyces cerevisiae
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Alternative names
- Nup84p antibody
Images
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All lanes : Anti-NUP84 antibody (ab122883) at 2 µg/ml (incubation time: 1 hour)
Lane 1 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - knock out
Lane 2 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - wild type
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 84 kDa
Datasheets and documents
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Datasheet download
References (2)
ab122883 has been referenced in 2 publications.
- Lord CL & Wente SR Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress. J Cell Biol 219:N/A (2020). PubMed: 33053148
- Chung DK et al. Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process. Nat Commun 6:7742 (2015). PubMed: 26205667