Anti-NUP88 antibody (ab79785)
Key features and details
- Rabbit polyclonal to NUP88
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NUP88 antibody
See all NUP88 primary antibodies -
Description
Rabbit polyclonal to NUP88 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human NUP88 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90188) -
Positive control
- This antibody gave a positive signal in HeLa whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa). |
Target
-
Function
Essential component of nuclear pore complex. -
Tissue specificity
Ubiquitous. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nuclear pore complex. - Information by UniProt
-
Database links
- Entrez Gene: 4927 Human
- Entrez Gene: 19069 Mouse
- Entrez Gene: 113929 Rat
- Omim: 602552 Human
- SwissProt: Q99567 Human
- SwissProt: Q8CEC0 Mouse
- SwissProt: O08658 Rat
- Unigene: 584784 Human
see all -
Alternative names
- 88 kDa nucleoporin antibody
- karyoporin antibody
- Nuclear pore complex protein 88 antibody
see all
Images
-
Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Nuclear pore complex protein Nup88 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (83 kDa).
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab79785 has been referenced in 6 publications.
- Iyer SR et al. Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle. Exp Gerontol 150:111338 (2021). PubMed: 33862137
- Lange J et al. Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons. Front Cell Neurosci 15:742763 (2021). PubMed: 34658796
- Fichtman B et al. Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy. Am J Hum Genet 105:48-64 (2019). PubMed: 31178128
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
- Grima JC et al. Mutant Huntingtin Disrupts the Nuclear Pore Complex. Neuron 94:93-107.e6 (2017). PubMed: 28384479
- Sugiyama K et al. Calpain-Dependent Degradation of Nucleoporins Contributes to Motor Neuron Death in a Mouse Model of Chronic Excitotoxicity. J Neurosci 37:8830-8844 (2017). PubMed: 28821644