Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)


  • Product name
    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker
    See all NUP98 primary antibodies
  • Description
    Rabbit monoclonal [EPR6678] to NUP98 - Nuclear Pore Marker
  • Specificity
    ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NUP98 aa 1600-1700. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
    Database link: P52948

  • Positive control
    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 188 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
    • Involvement in disease
      Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
      Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
      Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
      Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
      Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • Sequence similarities
      Belongs to the nucleoporin GLFG family.
      Contains 1 peptidase S59 domain.
    • Domain
      Contains G-L-F-G repeats.
    • Post-translational
      Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
    • Cellular localization
      Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
    • Information by UniProt
    • Database links
    • Alternative names
      • 96 kDa nucleoporin antibody
      • 98 kDa nucleoporin antibody
      • ADAR2 antibody
      • ADIR2 antibody
      • GLFG-repeat containing nucleoporin antibody
      • Nuclear pore complex protein Nup96 antibody
      • Nuclear pore complex protein Nup98 Nup96 antibody
      • Nucleoporin 98kD antibody
      • nucleoporin 98kDa antibody
      • Nucleoporin Nup96 antibody
      • Nucleoporin Nup98 antibody
      • NUP196 antibody
      • NUP96 antibody
      • Nup98 antibody
      • Nup98-Nup96 antibody
      • NUP98_HUMAN antibody
      see all

    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker images

    • All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco-2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 188 kDa
    • ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    References for Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

    This product has been referenced in:
    • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Mouse Tissue sections (postnatal 60days brain)
    Yes - 0.4%triton 30min
    postnatal 60days brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. liang zhang

    Verified customer

    Submitted Feb 03 2017


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