The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).
Use a concentration of 10 µg/ml.
RelevanceNUPL1 is a member of the nucleoporin family. It is a component of the nuclear pore complex, a complex required for trafficking across the nuclear membrane.
Cellular localizationNucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.