Overview

  • Product name
  • Description
    Mouse polyclonal to NUPL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NUPL1 protein (NP_054808.1)

  • Positive control
    • NUPL1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    NUPL1 is a member of the nucleoporin family. It is a component of the nuclear pore complex, a complex required for trafficking across the nuclear membrane.
  • Cellular localization
    Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.
  • Database links
  • Alternative names
    • KIAA0410 antibody
    • Nucleoporin like 1 antibody
    • Nucleoporin p58/p45 antibody
    • NUPL 1 antibody
    • OTTHUMP00000018146 antibody
    • OTTHUMP00000042323 antibody
    • PRO2463 antibody
    see all

Images

  • All lanes : Anti-NUPL1 antibody (ab69954) at 1/500 dilution

    Lane 1 : NUPL1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab69954 at 10µg/ml staining NUPL1 in Hela cells by Immunocytochemistry/Immunofluorescence.

References

ab69954 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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