Overview

  • Product nameAnti-NUR77 antibody
    See all NUR77 primary antibodies
  • Description
    Rabbit polyclonal to NUR77
  • Specificityab73982 detects endogenous levels of total NUR77.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human NUR77 around the phosphorylation site of serine 351 (L-P-SP-K-P).

  • Positive control
    • Human colon carcinoma tissue. Extracts from COS7 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab73982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • FunctionOrphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
  • Tissue specificityFetal muscle and adult liver, brain and thyroid.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early response protein NAK1 antibody
    • GFRP 1 antibody
    • GFRP antibody
    • GFRP1 antibody
    • Growth factor inducible nuclear protein N10 antibody
    • Growth Factor Inducible Nuclear Protein NP10 antibody
    • Growth Factor Response Protein 1 antibody
    • Hbr1 antibody
    • HMR antibody
    • Hormone Receptor antibody
    • MGC9485 antibody
    • N10 antibody
    • N10 nuclear protein antibody
    • NAK 1 antibody
    • NAK1 antibody
    • Nerve growth factor IB nuclear receptor variant 1 antibody
    • NGFIB antibody
    • NP 10 antibody
    • NP10 antibody
    • NR4A1 antibody
    • NR4A1_HUMAN antibody
    • Nuclear hormone receptor NUR/77 antibody
    • Nuclear Hormone Receptor TR3 antibody
    • Nuclear receptor subfamily 4 group A member 1 antibody
    • NUR77 antibody
    • NUR77, mouse, homolog of antibody
    • Orphan nuclear receptor HMR antibody
    • Orphan nuclear receptor NR4A1 antibody
    • Orphan nuclear receptor TR3 antibody
    • Orphan receptor tr3 antibody
    • Receptor NGFIB antibody
    • ST 59 antibody
    • ST-59 antibody
    • ST59 antibody
    • Steroid receptor TR3 antibody
    • Testicular receptor 3 antibody
    • TR 3 antibody
    • TR3 antibody
    • TR3 orphan receptor antibody
    see all

Anti-NUR77 antibody images

  • All lanes : Anti-NUR77 antibody (ab73982) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ab73982, at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-NUR77 antibody (ab73982)

ab73982 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12 cells)
Loading amount 30 µg
Specification PC12 cells
Treatment 300nM dbcAMP for 1hr
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"