Overview

  • Product nameAnti-NUR77 antibody - ChIP Grade
    See all NUR77 primary antibodies
  • Description
    Rabbit polyclonal to NUR77 - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, ChIP, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    TSTKARSGAPGGSEGR

    , corresponding to amino acids 251-266 of Human NUR77.

  • Positive control
    • Molt4 or NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21726557
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa.

Target

  • FunctionOrphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
  • Tissue specificityFetal muscle and adult liver, brain and thyroid.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early response protein NAK1 antibody
    • GFRP 1 antibody
    • GFRP antibody
    • GFRP1 antibody
    • Growth factor inducible nuclear protein N10 antibody
    • Growth Factor Inducible Nuclear Protein NP10 antibody
    • Growth Factor Response Protein 1 antibody
    • Hbr1 antibody
    • HMR antibody
    • Hormone Receptor antibody
    • MGC9485 antibody
    • N10 antibody
    • N10 nuclear protein antibody
    • NAK 1 antibody
    • NAK1 antibody
    • Nerve growth factor IB nuclear receptor variant 1 antibody
    • NGFIB antibody
    • NP 10 antibody
    • NP10 antibody
    • NR4A1 antibody
    • NR4A1_HUMAN antibody
    • Nuclear hormone receptor NUR/77 antibody
    • Nuclear Hormone Receptor TR3 antibody
    • Nuclear receptor subfamily 4 group A member 1 antibody
    • NUR77 antibody
    • NUR77, mouse, homolog of antibody
    • Orphan nuclear receptor HMR antibody
    • Orphan nuclear receptor NR4A1 antibody
    • Orphan nuclear receptor TR3 antibody
    • Orphan receptor tr3 antibody
    • Receptor NGFIB antibody
    • ST 59 antibody
    • ST-59 antibody
    • ST59 antibody
    • Steroid receptor TR3 antibody
    • Testicular receptor 3 antibody
    • TR 3 antibody
    • TR3 antibody
    • TR3 orphan receptor antibody
    see all

Anti-NUR77 antibody - ChIP Grade images

  • Western blot analysis of NUR77 in 15 ugs of NIH3T3 cell lysate using ab13851 at 1:500 dilution.

References for Anti-NUR77 antibody - ChIP Grade (ab13851)

This product has been referenced in:
  • Yu Y  et al. The orphan nuclear receptor Nur77 inhibits low shear stress-induced carotid artery remodeling in mice. Int J Mol Med 36:1547-55 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 26498924) »
  • Ding LJ  et al. FSH acts on the proliferation of type A spermatogonia via Nur77 that increases GDNF expression in the Sertoli cells. FEBS Lett 585:2437-44 (2011). ICC/IF, WB ; Human, Mouse . Read more (PubMed: 21726557) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (DO11.10 thymocyte hybridoma)
Loading amount 25 µg
Specification DO11.10 thymocyte hybridoma
Treatment PMA/Ionomycin
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jonathan Rud

Verified customer

Submitted Dec 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293T cells over expressing Nur77)
Total protein in input 25 µg
Specification HEK 293T cells over expressing Nur77
Treatment transfected with Nur77 expression construct
Immuno-precipitation step Protein A/G
Username

Dr. Jonathan Rud

Verified customer

Submitted Dec 30 2009

Thank you for filling out our technical help questionnaire. The non-specific band you are seeing may very well be NUR77. The following reference describes the appearance of the protein in Western blots at sizes ranging from 63 - 88 kD, due to post-...

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Thank you for your enquiry. Ab13851 - Rabbit polyclonal to NUR77 - was generated using the following peptide: TSTKARSGAPGGSEGR, corresponding to amino acids 251-266 of Human NUR77. This antibody cross-reacts with human and mouse and is predicted (it ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"