Overview

  • Product nameAnti-NUR77 antibody [EPR3209]
    See all NUR77 primary antibodies
  • Description
    Rabbit monoclonal [EPR3209] to NUR77
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NUR77 (N terminal).

  • Positive control
    • SH-SY5Y cell lysate and HepG2 cells and lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionOrphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
    • Tissue specificityFetal muscle and adult liver, brain and thyroid.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early response protein NAK1 antibody
      • GFRP 1 antibody
      • GFRP antibody
      • GFRP1 antibody
      • Growth factor inducible nuclear protein N10 antibody
      • Growth Factor Inducible Nuclear Protein NP10 antibody
      • Growth Factor Response Protein 1 antibody
      • Hbr1 antibody
      • HMR antibody
      • Hormone Receptor antibody
      • MGC9485 antibody
      • N10 antibody
      • N10 nuclear protein antibody
      • NAK 1 antibody
      • NAK1 antibody
      • Nerve growth factor IB nuclear receptor variant 1 antibody
      • NGFIB antibody
      • NP 10 antibody
      • NP10 antibody
      • NR4A1 antibody
      • NR4A1_HUMAN antibody
      • Nuclear hormone receptor NUR/77 antibody
      • Nuclear Hormone Receptor TR3 antibody
      • Nuclear receptor subfamily 4 group A member 1 antibody
      • NUR77 antibody
      • NUR77, mouse, homolog of antibody
      • Orphan nuclear receptor HMR antibody
      • Orphan nuclear receptor NR4A1 antibody
      • Orphan nuclear receptor TR3 antibody
      • Orphan receptor tr3 antibody
      • Receptor NGFIB antibody
      • ST 59 antibody
      • ST-59 antibody
      • ST59 antibody
      • Steroid receptor TR3 antibody
      • Testicular receptor 3 antibody
      • TR 3 antibody
      • TR3 antibody
      • TR3 orphan receptor antibody
      see all

    Anti-NUR77 antibody [EPR3209] images

    • Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
      Blocking/Diluting buffer 5% NFDM/TBST
    • All lanes : Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 64 kDa
      Observed band size : 64 kDa
    • Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Mouse pituitary at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
      Blocking/Diluting buffer 5% NFDM/TBST
    • Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Rat brain at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
      Blocking/Diluting buffer 5% NFDM/TBST

    References for Anti-NUR77 antibody [EPR3209] (ab109180)

    This product has been referenced in:
    • Liu NA  et al. Cyclin E - mediated human proopiomelanocortin regulation as a therapeutic target for Cushing disease. J Clin Endocrinol Metab N/A:jc20151606 (2015). WB ; Human . Read more (PubMed: 25942479) »
    • Yu Y  et al. The orphan nuclear receptor Nur77 inhibits low shear stress-induced carotid artery remodeling in mice. Int J Mol Med 36:1547-55 (2015). WB ; Rat . Read more (PubMed: 26498924) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your email.
    Unfortunately we are not able to provide the immunogen peptide for this particular antibody, as the laboratory providing this antibody does not have it available. I am very sorry.
    In regards to positive control, I ca...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"