Purification notesab54366 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 66 kDa).
Use at an assay dependent dilution.
FunctionTranscriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
Tissue specificityExpressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Developmental stageRapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.