Overview

  • Product nameAnti-Nurr1 antibody
    See all Nurr1 primary antibodies
  • Description
    Rabbit polyclonal to Nurr1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH-conjugated synthetic peptide in the N-terminal region of human Nurr1

  • Positive control
    • Mouse brain tissue lysate; human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionTranscriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
  • Tissue specificityExpressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stageRapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HZF 3 antibody
    • HZF3 antibody
    • Immediate-early response protein NOT antibody
    • Intermediate early receptor protein antibody
    • NGFI B/nur77 beta type transcription factor homolog antibody
    • NOT antibody
    • Nr4a2 antibody
    • NR4A2_HUMAN antibody
    • nuclear receptor of T cells antibody
    • nuclear receptor related 1 antibody
    • Nuclear receptor subfamily 4 group A member 2 antibody
    • Nur related protein 1 homolog antibody
    • nur related protein-1, human homolog of antibody
    • Nurr 1 antibody
    • Orphan nuclear receptor NR4A2 antibody
    • Orphan nuclear receptor NURR1 antibody
    • RNR 1 antibody
    • RNR1 antibody
    • T cell nuclear receptor NOT antibody
    • T-cell nuclear receptor NOT antibody
    • TINUR antibody
    • Transcriptionally inducible nuclear receptor antibody
    • Transcriptionally inducible nuclear receptor related antibody
    • Transcriptionally inducible nuclear receptor related 1 antibody
    • Transcriptionally-inducible nuclear receptor antibody
    see all

Anti-Nurr1 antibody images

  • Anti-Nurr1 antibody (ab60149) at 1/100 dilution + mouse brain tissue lysate

    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 antibody (N-term) (ab60149), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-Nurr1 antibody (ab60149)

ab60149 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (Coronal head sections 12 um thickness (cryostat))
Specification Coronal head sections 12 um thickness (cryostat)
Fixative Paraformaldehyde 2% 3hrs or 4% 2 hrs or 4% 3 hrs
Permeabilization Yes - with and w/o Ag retrieval
Blocking step (agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted May 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"